Deciphering the effects of kosmotrope and chaotrope salts during aqueous two phase extraction (ATPE) of polyphenolic compounds and glycoalkaloids from the leaves of a nutraceutical plant, Solanum retroflexum, with the aid of UPLC-QTOF-MS

Abstract Solanum plants (Solanaceae) are renowned source of nutraceuticals and have widely been explored for their phytochemical constituents. This work investigated the effects kosmotropic chaotropic salts on number phytochemicals extracted from leaves a nutraceutical plant, retroflexum, analyzed ultra-performance liquid chromatography hyphenated to quadrupole time flight mass spectrometer (UPLC-QTOF-MS) detector. Here, total 20 different compounds were putatively characterized. The majority identified polyphenols glycoalkaloids. Another compound, caffeoyl malate was first in this plant. Glycoalkaloids such as solanelagnin, solamargine, solasonine, ?-solanine (I) (II) found be by almost all used herein. Kosmotrope salts, overall, more efficient extracting polar with 4 polyphenolic compared chaotropes. Chaotropes generally selective extraction less (glycoalkaloids) 3 than kosmotropes. chaotrope kosmotrope that most metabolites NaCl Na 2 SO , respectively, 12 each salt. demonstrated comprehensive metabolome S. what previously reported same can achieved application kosmotropes chaotropes extractants aid Aqueous Two Phase Extraction approach. best-performing or NaCl, could potentially applied commercial scale, meet ever-growing demand studied metabolites. technique simultaneous multiple which metabolomics.